A comparison of yeast mannan mutants by electron microscopy.

Genetic control of yeast mannan structure. Isolation and characterization of mannan mutants.

The yeast Saccharomyces cerevisiae X2180, the strain most commonly used for genetic studies on yeasts, was treated with the mutagen ethyl methanesulfonate, and mutants defective in the synthesis of cell wall mannan were isolated. The initial selection of mutants was made on the basis of their failure to agglutinate with antiserum that was specific for the mannotetraose side chain of the mannan,...

Supplemental Chromium Yeast and / orMannan Oligosaccharides in Growing Quail Diets

To evaluatethe effects of supplementing diets of growing Japanese quails (n=450; 7 days old) with chromium (Cr) yeast and / or mannan oligosaccharides (MOS) on productive performance, carcass traits and the immune system are reported. Diets were a basal diet (control) or the basal diet supplemented with 600 mg Cr-yeast/kg (Cr 1), 1200 mg Cr-yeast/kg (Cr 2), 1.0 g MOS/kg, Cr 1 + MOS or Cr 2 + MO...

Identification of an additional protein involved in mannan biosynthesis

Galactomannans comprise a β-1,4-mannan backbone substituted with α-1,6-galactosyl residues. Genes encoding the enzymes that are primarily responsible for backbone synthesis and side-chain addition of galactomannans were previously identified and characterized. To identify additional genes involved in galactomannan biosynthesis, we previously performed deep EST profiling of fenugreek (Trigonella...

Detection of rotavirus by Latex Agglutination Test (Rotales); Comparison with Electron Microscopy and Complement Fixation Test

High-resolution x-ray diffraction microscopy of specifically labeled yeast cells.

X-ray diffraction microscopy complements other x-ray microscopy methods by being free of lens-imposed radiation dose and resolution limits, and it allows for high-resolution imaging of biological specimens too thick to be viewed by electron microscopy. We report here the highest resolution (11-13 nm) x-ray diffraction micrograph of biological specimens, and a demonstration of molecular-specific...